The Seagulls, who are seventh in the Premier League, welcome an Ajax club who are having plenty of trouble themselves.
Yesterday they sacked their manager Maurice Steijn in the wake of a 4-3 defeat at Utrecht that left the Dutch giants sitting one point off the bottom of the table with just one win from their first seven games.
Ajax have also been hit by crowd trouble this season with their derby against Feyenoord forced to finish behind closed doors, while their loss at Utrecht was also temporarily suspended late on.
Brighton have had no such trouble but the injuries are concern for head coach De Zerbi, ahead of a tricky fixture schedule in the Premier League and Europa League, where they face Ajax, Fulham, Everton, Ajax again and Sheffield United before mid-November.
Here's the full list of Albion players who have been ruled out of Thursday's clash against Ajax...
1.
Brighton's Italian head coach Roberto De Zerbi has injury concerns ahead of the Europa League fixture against Ajax Photo: OLI SCARFF
2. Danny Welbeck - muscular issue
Forced off in the first half against Man City last Saturday and will miss the Ajax clash at least. Fulham on Sunday is also a major doubt Photo: Charlotte Tattersall
3. Solly March - knee
Looks set for a lengthy spell on the sidleines after twisting his right knee late on at Man City Photo: OLI SCARFF
4. Tariq Lamptey - muscular
The flying full back has not played since impressing at Marseille. De Zerbi said after City that Lamptey will not feature against Ajax Photo: Mike Hewitt